SECTION TWO 



C. J.S.B. Stain No. i 



(i) Prepare the solution in ac- 

 cordance with notes {b) and 

 {c) above 



or 



(ii) J.S.B. stain (Michrome) pow- 

 der . . . . . . 0-45 gm. 



Distilled water . . . . 100 ml. 



Techtiique: 



1. Take three Coplin jars; fill one with solution A, another 

 with solution B, and the third with solution C. 



2. Dip smears in the jar containing solution A (eosin) for one 

 to three seconds. 



3. Remove excess eosin by dipping the smears into solution B 

 (buffered wash water). 



4. Immediately transfer to solution C and leave therein for 

 forty to forty-five seconds. 



5. Wash by dipping, in the same jar of solution B as used at 

 stage 3, three or four times. 



6. Dry and examine under the oil immersion objective. 



Results: 



Similar to Giemsa stained preparations. 



Notes: 



{a) Manwell (1945) concluded that this stain was found to be 

 superior in most respects to any of the commonly used processes 

 for staining blood and blood parasites. The authors (Jaswant 

 Singh, A. P. Ray and C. P. Nair) state that J.S.B. stain has 

 completely replaced Leishman and Giemsa stains in their labor- 

 atories in India. However, my own experience is that Giemsa, 

 May-Griinwald, Leishman and Wright, all of which are used in 

 vast quantities, appear to be favoured by a large majority of the 

 biologists in many countries. 



{b) It is stated that solutions of J.S.B. stain are not difficult to 

 make up, are relatively inexpensive, and keep well for weeks or 

 months, even in hot weather. 



255 



