STAINING, PRACTICAL AND THEORETICAL 



(c) The preparations are said to be somewhat less resistant to 

 fading than the usual blood stains such as Giemsa, Wright, etc., 

 but they will stand much more exposure to light than they would 

 ordinarily receive. 



(d) For more detailed information, readers are referred to the 

 original papers, particularly the second one cited below. 



References : 



Jaswant, Singh & Bhattacharji, L. M. (1944). 

 Jaswant, Singh, Ray, A. P. & Nair, C. P. (i953)- 

 Manwell, R. D. (1945). 



KH STAINS 

 (Edward Gurr) 



For keratin, vaginal epithelium, etc. 



Solutions required: 



A. Trichloric acid, 1% in 80% alcohol 



B. KHi or KH8 or KH9 stain 



Technique: 



1. Fix material in solution A and embed in paraffin wax as 

 usual. 



2. Fix sections to slides and carry through to 70% alcohol in 

 the usual way. 



3. Rinse briefly with distilled water. 



4. Immerse in the KH staining solution for five minutes. 



5. Rinse in 70% alcohol. 



6. Rinse in 90% alcohol. 



7. Dehydrate with absolute alcohol. 



8. Clear in xylol. 



9. Mount in D.P.X. or Clearmount or Cristalite. 



Results: 



