STAINING, PRACTICAL AND THEORETICAL 



drying may be very considerably shortened by placing the slide 

 in an incubator at 37° C. 



2. Remove the haemoglobin by placing the film face down- 

 wards in a dish of distilled water at room temperature. 



3. Fix in acid alcohol for five to fifteen minutes. 



4. Wash well with distilled water ; blot gently and dry in air. 



5. Stain in accordance with the method described above for 

 thin films. 



Results: 



Similar to Wright's stain. 

 Reference: Leishman, W. B. (1901). 



LEAD HAEMATOXYLIN - ACID FUCHSIN 

 (M. A. MacConaill) 



A differential stain for neurokeratin 



Solutions required: 



A. Lead nitrate 

 Glacial acetic acid 

 Acid fuchsin 

 Water 



B. Haematoxylin 

 Acetic acid 4% aqueous 



C. Solution A 

 Solution B 



2gm. 

 8 ml. 

 0-5 gm. 

 92 ml. 



I gm. 

 100 ml. 



I volume 

 I volume 



Note: This solution should be prepared as and 

 ' when required : it deteriorates after one day. 



D. Ammonium acetate 6% aqueous 10 ml. 

 Ammonium molybdate, saturated, 



aqueous 70 ml. 



Water 80 ml. 



Note: All the above solutions must be made with- 

 out the application of heat. Tap water may be used : 

 the solutions must be filtered. 



258 



