STAINING, PRACTICAL AND THEORETICAL 



3. Take sections down to water as usual. 



4. Stain in solution B for three to five minutes. 



5. Wash briefly in water. 



6. Counterstain for thirty to sixty sections in the safranin solu- 

 tion. 



7. Rinse briefly with water. 



8. Dehydrate as usual. 



9. Clear in xylol. 



10. Mount in Clarite or other synthetic mountant such as 

 D.P.X., Clearmount, etc. 



Results: 



Haemoglobin, dark blue-green; background light pink. 



Note: Blood and tissue smears fixed with methyl alcohol may 

 also be stained by applying the stains as prescribed above. 



References : 



Dunn, R. C. (1946). 

 Gurr, E. (1958a). 



LEVADIXrS STAIN 

 For Treponema pallidum in sections 



Technique: 



Tissues about i mm. thick should be fixed for twenty-four hours 

 in 10% formalin and embedded in paraffin wax after staining. 



1. After rinsing tissues in tap water, immerse in 95% alcohol 

 for twenty-four hours. 



2. Immerse in distilled water until the tissue sinks to the bottom 

 of the jar. 



262 



