STAINING, PRACTICAL AND THEORETICAL 



LIPID CRIMSON METHOD 

 For lipids 



Reagents required: 



A. Lipid crimson (Michrome No. 931) i gm. 

 Alcohol, 70% . . . . . . 100 ml. 



B. Haematoxylin (Ehrlich) 



Technique: 



1. Fix material in 10% formaldehyde and cut sections on a 

 freezing microtome, or use freehand sections. 



2. Stain in reagent A for ten to fifteen minutes. 



3. Wash rapidly in 50% alcohol. 



4. Wash in distilled water. 



5. Stain in the haematoxylin solution for five to ten minutes. 



6. " Blue " in tap water or in 1% lithium carbonate solu- 

 tion. 



7. Wash in distilled water. 



8. Mount in Aquamount. 



Results: 



Lipids: brilliant crimson. Nuclei: blue to bluish black. 



Notes: 



(a) This method gives better results than Sudan 3 or Sudan 4 

 or Scarlet R, the colour being much more intense and the smaller 

 lipid-particles or droplets are readily visible. 



(b) Bodman (i960) employs lipid crimson in gel electrophoresis 

 for staining lipo-protein complexes. 



Reference: Gurr, E. (1958a). 



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