SECTION TWO 



LORRAIN SMITH - DIETRICH STAIN 

 For lipids 



Solutions required: 



A. Potass, dichromate 5% aqueous. 



Haematoxylin [Kultschitzky) 



B. Haematoxylin 10% in absolute 



alcohol (ripened three months 



or longer) . . . . . . 10 ml. 



Acetic acid 2% aqueous . . 90 ml. 



C. Potass, ferricyanide .. .. 2-5 gm. 

 Borax 2% aqueous , . . . 100 ml. 



Technique: 



Material is fixed in 10% formalin and frozen sections are em- 

 ployed. 



1. Mordant sections twenty-four to forty-eight hours in Solu- 

 tion A at 37° C. ; then wash thoroughly in distilled water. 



2. Immerse in Solution B at 37° C. for four to five hours; then 

 wash in distilled water. 



3. Differentiate overnight in Solution C. 



4. Wash thoroughly in distilled water; drain and mount in 

 Aquamount. 



Results: 



Lipids: blue-black. 



References: 



Dietrich, A. (1910). 

 Mallory, F. B. (1938), p. 123. 

 Smith, J. Lorrain & Mair, W. (1908). 



LUGOL'S IODINE 

 For the identification of glycogen in tissues 



Solution required: 

 Lugol's iodine. 



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