STAINING, PRACTICAL AND THEORETICAL 



2. Cut sections at 4 to 5^ and fix them to slides. 



3. Remove paraffin wax with xylol. 



4. Carry to distilled water, through the usual descending 

 grades of alcohol. 



5. Immerse in solution A for one and a half hours. 



6. Wash well in tap water. 



7. Wash in 70% alcohol. 



8. Immerse in the luxol fast green solution for one hour, in a 

 covered jar, to prevent evaporation of the alcohol. 



9. Rinse in 95% alcohol. 



10. Dehydrate with absolute alcohol. 



11. Mount in Clearmount or Michrome mountant or proceed 

 as follows: 



12. Rinse in xylol. 



13. Mount in Clearmount, Michrome mountant, D.P.X., or 

 Emexel. 



Results: 



When viewed with dark contrast medium objectives, nucleoli, 

 nuclear chromatin and nuclear membranes appear black against a 

 background of green cytoplasm, while certain cytoplasmic struc- 

 tures, such as the secretion granules of pancreatic acinar cells, 

 appear black also. The contrast of epithelial cells although 

 increased was not of the same high degree as in the case of luxol 

 fast blue (see page 273). 



Notes: 



The authors found that 10% formalin, Bouin and Zenker's 

 fixative gave equally good results. The materials used were 

 salivary gland, pancreas, lymph node, liver, spleen, uterus, and 

 ileum. It is suggested by the authors that the technique might 

 also be applied with advantage in the study of a wider variety of 

 organs. Other fixatives might also be used, besides the three 

 mentioned above as having been tried by the authors. 



Reference: Wood, M. L. & Green, J. A. (19586). 



276 



