STAINING, PRACTICAL AND THEORETICAL 



guinea-pig, rabbit, cat, monkey, chimpanzee, gorilla and man), 

 and that with suitable counterstains Luxol fast blue will give ex- 

 cellent preparations of cochlea, adrenals and numerous extraneural 

 tissues. 



References : 



Kluver, H. & Barrera, Elizabeth (1953). 

 Kliiver, H, & Barrera, Elizabeth (1954). 



LUXOL FAST SCARLET C 



As a stain for phase contrast microscopy for increasing 

 contrast of cellular structures, etc. 



Solutions required: 



A. Tannic acid, 1% aqueous 



B. Luxol fast scarlet C 2% in 95% 



ethyl alcohol 



Technique: 



1. Fix material in any of the following fixatives: Bouin, Helly, 

 Regaud, Zenker, Susa-Heidenhain, Flemming's fluid, or 10% 

 formalin. 



2. Embed in paraffin wax in the usual way, and cut sections at 



4 to 5/x. 



3. Fix sections to slides, dewax, and carry through the usual 

 descending grades of alcohol into water. 



4. Immerse in the tannic acid solution for six to twelve hours. 



5. Rinse in tap water. 



6. Wash with 70% alcohol. 



7. Immerse in the Luxol fast scarlet solution for one hour, in 

 a well-closed staining jar to prevent evaporation of the alcohol. 



8. Dehydrate in absolute alcohol, clear in xylol and mount in 

 D.P.X., Cristalite, Clearmount, or Emexel. 



Results: 



The sections, observed with dark contrast medium objectives, 

 show cellular and nuclear membranes, chromatin and cytoplasmic 

 structures, black against a light red background. Secretion 

 granules and mitochondria of the pancreas and salivary glands 

 are stated by the author (J. A. Green) to be well defined when 

 their fixation has been satisfactory; while renal tubule cell struc- 

 ture, epithelial cell structure, cells in lymph nodes, and reticular 



280 



