STAINING, PRACTICAL AND THEORETICAL 



B. Formic acid io% aqueous. 



C. Iodine 0-5% in 70% alcohol. 



D. Sodium thiosulphate 075% in 10% alcohol. 



E. Harris haematoxylin. 



F. Lithium carbonate satd. aqueous. 



G. Acid fuchsin acetic (Mallory) 0-5% aqueous. 

 H, Phosphomolybdic acid 1% aqueous. 



I. Mallory's Aniline blue-orange G. 

 J. Carbol xylol. 



Technique : 



1. Pituitary gland together with bone to which it is attached is 

 fixed in solution A for 4-8 hours, or overnight in a refrigerator. 



2. Wash in running tap water for 8 to 10 hours. 



3. Decalcify by immersing in 10% formic acid for 24 hours. 



4. Wash in running tap water for 2 to 4 hours. 



5. Dehydrate, clear and embed in paraffin wax 56 to 58° C. in 

 the usual way. 



6. Cut sections about ^n in thickness and mount on slides with 

 glycerine albumen. 



7. Dry the slides thoroughly in an oven at 56° C. for one to two 

 hours, or overnight at room temperature. 



8. Remove wax with xylol; then wash with two changes of 

 absolute alcohol. 



9. Wash with 90% followed by 70% alcohol. 



10. Immerse for half to two minutes in solution C (Iodine). 



1 1 . Wash well with water. 



12. Immerse in solution D (thiosulphate) for half to two minutes 

 or until the natural colour of the sections is restored. 



13. Wash well with tap water. 



14. Rinse in distilled water. 



15. Stain in Harris haematoxylin for 2-3 minutes. 



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