SECTION TWO 



1 6. Rinse in'tap water. 



17. Blue in the lithium carbonate solution for i minute. 



18. Rinse well in distilled water. 



19. Stain in the acid fuchsin solution for 1-2 minutes. 



20. Rinse quickly in tap water. 



21. Immerse in the phosphomolybdic acid solution for 2-5 

 minutes. 



22. Without washing, pass the slides directly into Mallory's 

 Aniline blue - orange G and leave therein for 1-2 hours. 



23. Differentiate with 95% alcohol, controlling by examination 

 under the microscope until the blue granules are intense, but the 

 fuchsinophil granules are clearly visible, 



24. Rinse with absolute alcohol. 



25. Rinse with carbol xylol. 



26. Immerse in two changes of xylol for 10 minutes in each. 



27. Mount in Clearmount of D.P.X. 



Results: 



The nuclei of all cells are stained dark blue black. Granules of 

 basophils: blue. Acidophil granules: brilliant red. Non-granular 

 cytoplasm of chromophobes : light grey. Erythrocytes : orange to 

 red. Bone : intense blue. 



Reference: Gude, William D. (1953). 



MALLORY STAIN - HAEMATOXYLIN (Ehrlich) 

 For Negri bodies in sections of brain 



Solutions required: 



A. Ehrlich haematoxylin. 



B. Orange G. . . . . . . . . 0-5 gm. 



Phosphotungstic acid, saturated 

 aqueous .. .. .. .. 100 ml. 



K* 285 



