STAINING, PRACTICAL AND THEORETICAL 



C. Acid fuchsin 

 Phosphotungstic acid 

 Acetic acid i % 



D. Phosphotungstic acid 

 Phosphomolybdic acid 

 Picric acid, saturated, aqueous 

 Absolute alcohol 



E. Aniline blue, aqueous 

 Distilled water 

 Glacial acetic acid . . 



0-5 gm. 

 0-5 gm. 

 100 ml. 



2 gm. 

 2 gm. 

 70 ml. 

 30 ml. 



1 gm. 

 98 ml. 



2 ml. 



Technique: 



1 . Fix paraffin sections to slides ; dewax and take down to dis- 

 tilled water in the usual way, 



2. Stain in Ehrlich haematoxylin for five minutes. 



3. Blue in tap water, or in lithium carbonate solution, for two 

 minutes, 



4. Rinse in distilled water. 



5. Stain for one minute in the orange G solution. 



6. Wash in tap water until only the erythrocytes are stained 

 yellow. 



7. Rinse in distilled water, 



8. Stain for ten minutes in the acid fuchsin solution. 



9. Rinse in distilled water, 



10. Differentiate for five minutes in solution D. 



1 1 . Rinse in distilled water. 



12. Rinse in 1% acetic acid, 



13. Dehydrate, clear and mount. 



Results : 



Negri bodies : purplish red with blue granulations. Cytoplasm 

 of neurones : bluish. Nucleoli : dark purple. Erythrocytes : yellow. 



Reference: Zlotnik, I. (1953). 



286 



