SECTION TWO 



MALLORY HEIDENHAIN STAIN - HAEMATOXYLIN 



For the differentiation of cells in the hypophysis and in 



the pancreatic islets 



Solutions required: 



A. Potassium permanganate, 0-3% 



aqueous 



B. Sulphuric acid, 3% aqueous 



C. Potassium metabisulphate, 4% 



aqueous 



D. Gomoris chrome haematoxylin 



Chrome alum, 3% aqueous . • 5° "^1- 

 Haematoxylin, 1% aqueous . . 5° ^nl- 

 Potassium dichromate, 5% 



aqueous . • • • • • ^ "^^• 



Solution B (sulphuric acid, 3%) . . 3*35 ml. 



E. Sulphuric acid 1% in 70% alcohol 



F. Jane E. Cason's modified Mallory- 



Heidenhain stain 

 Acidfuchsin 3 g"^- 



Phosphotungstic acid 

 Orange G 



Aniline blue, aqueous 

 Distilled water 



Technique i 



I gm. 



2gm. 



I gm. 



200 ml. 



For hypophysis 



I. Slice large hypophysis, such as human, into three pieces in 

 the planes for future sectioning; or dissect small ones from the 

 sella turcica (these need not be divided). ^ 



2 Fix for four to twenty-four hours, dependmg on the size ot 

 the specimen, in Susa-Heidenhain, or for one to three days m 

 formalin-sublimate, or in 10% formahn. 



3 Embed in paraffin wax as usual and cut sections at 3 to 5/x. 

 4. Fix sections to slides and carry through to distilled water, 



but if a mercury-containing fixative has been used, treat for the 

 removal of mercurial precipitate before transferring to water. 



289 



