SECTION TWO 



Notes: 



{a) The authors (Rona & Morvay, of the Cancer Research 

 Department, Medical University, Budapest, Hungary) state that 

 the production of clear pictures of the cellular granules of the 

 hypophysis and especially of the pancreatic islets is one of the 

 most difficult histological tasks. Nearly all the recommended 

 methods are lengthy and complicated and the accepted procedures 

 often give unsatisfactory and variable results. These difficulties 

 led to the development of the above techniques, which are con- 

 sidered to be reliable and relatively simple. 



{b) The methods facilitate the study of the cell types in the 

 hypophysis or in the pancreatic islets, and make it possible to 

 estimate the granule content of the cells. 



{c) The method works on human autopsy material provided 

 fixation of hypophysis takes place within twenty-four hours, and 

 pancreas within twelve hours, of death. 



{d) For further information readers should consult the authors' 

 original paper. 



Reference: R6na, G. & Morvay, I. (1956). 



MARSHALL RED - VICTORIA GREEN 

 A general stain, particularly suitable for class work 



Solutions required: 



A. Marshall red, saturated aqueous. 



B. Victoria green saturated in 70% alcohol. 



Technique : 



1. Fix sections to slides; dewax and take down to distilled 

 water in the usual manner. 



2. Stain in the Marshall red solution for twenty minutes. 



3. Rinse in distilled water. 



4. Stain in the Victoria green solution for half an hour. 



5. Rinse in 70% alcohol followed by 90% alcohol. 



6. Dehydrate with absolute alcohol. 



7. Clear in xylol and mount. 



291 



