SECTION TWO 



7. Rinse in distilled water. 



8. Differentiate in picric alcohol (Solution C above) controlling 

 by examination under the microscope, while the preparation is 

 still wet. 



9. Wash in running tap water for a minute or so. 



10. Stain for five minutes in the Ponceau fuchsin solution. 



1 1 . Rinse in distilled water. 



12. Differentiate in the phosphomolybdic acid solution for five 

 minutes. 



13. Add 0-5 ml. of the acetic aniline blue (Solution F above) to 

 the phosphomolybdic acid on the slide and mix by rocking the 

 slide gently. Allow this mixture to act for five minutes. 



14. Pour off excess liquid and rinse in distilled water. 



15. Immerse in phosphomolybdic acid solution again, for five 

 minutes. 



16. Transfer to 1% acetic acid and leave therein for five minutes. 



17. Wash in distilled water. 



18. Dehydrate in 95% alcohol, followed by absolute alcohol; 

 clear in xylol ; mount. 



Results: 



Collagen, deep blue. Neuroglia fibrils, red. Nuclei, black. 

 Argentaffin granules, black'^or red. 



Reference: Masson, P. (1929). 



MAXILON BLUE RL 

 For mucopolysaccharide structures 



Solutions required: 



A. Formol-calcium fixative (Baker, 



1946) {see page 226) 



B. Maxilon blue RL, 0-05% in dis- 



tilled water 



C. Tertiary butyl alcohol 



293 



