SECTION TWO 



not yet found confimiation by others (Glick, 1940). There is 

 some expermiental support, according to de Almeida, to Hempel- 

 mann's suggestion, since the binding strength of the chromotropes 

 to the dye varies with the acidic character of the radical involved, 

 in the order COOH < PO4 < SO4 (Bank & Bungenberg de 

 Jong, 1939). The author also states that in the work of Kelley 

 & Miller (1935) evidence can be found for the histological differ- 

 entiation between metachromatic staining due to nucleoproteins 

 and to mucins, and that there seems to be no reason why this 

 concept could not be extended to the various acid mucopoly- 

 saccharides. 



Reference: Almeida^ D. F. de (i960). 



MAY-GRUNWALD STAIN 



This stain may be used alone for the cytological examination 

 of blood smears but it is not suitable for parasites. The technique 

 is the same as that for Jenner stain (page 250). The chief use 

 of May-Griinwald stain is in conjunction with Giemsa stain 

 (page 251). 



Reference: May & Griinwald (1902). 



METHYL BLUE - EOSIN (Mann) 



For demonstrating the various types of cells in the anterior 



lobe of the pituitary and for the study of the relationship 



and development of the blood vessels 



Solution required: 

 Methyl Blue-Eosin (Mann's stain). 



Technique: 



I . Paraffin sections of tissues which have been fixed in a fluid con- 

 taining mercuric chloride are mounted on slides and treated by the 

 standard technique for the removal of mercuric precipitate. 



295 



