STAINING, PRACTICAL AND THEORETICAL 



2. Bring the sections down to distilled water; then stain for a 

 quarter of an hour to two hours (the longer time is required if it is 

 desired to demonstrate anterior lobe of pituitary). 



3. Wash and differentiate in tap water. 



4. Dehydrate rapidly with two changes of absolute alcohol. 



5. Clear in xylol ; mount in xylol balsam and examine under the 

 1 ow power, as the stain is too diffuse for critical high power work. 



Results: 



Nuclei are stained blue; karyosomes, dark blue; plasmosomes, 

 red; basophil cytoplasm, blue; oxyphil cytoplasm and oxyphil 

 granules, red. 



Note: Although this method is of considerable antiquity, it is 

 still used to some extent, and the above details are given to satisfy 

 requests received from time to time from biologists in various 

 countries for details of this technique. 



Reference: Mann, G. (1894). 



METHYL GREEN 

 For amyloid 



Solutiofi required: 

 Methyl green 0-3% aqueous 



Technique: 



1. Fix tissues in absolute alcohol or in 10% formalin and cut 

 frozen sections. 



2. Immerse in the methyl green solution for five to ten hours. 



3. Wash well in distilled water. 



4. Mount in neutral glycerine, or in aquamount. 



Results: 



Amyloid is stained reddish violet, while other tissue elements 

 are green. 



Reference: Cowdry, E. V. (1952). 



296 



