SECTION TWO 



METHYL VIOLET 6B 



For amyloid 



Solution required: 

 Methyl Violet 6B aqueous i%. 



Technique: 



1. Material should be fixed in alcohol and embedded in paraffin 

 wax (although frozen sections may be employed). 



2. Paraffin sections are fixed to slides and brought down to dis- 

 tilled water. 



3. Stain for two to five minutes in the methyl violet 6B solution. 



4. Rinse quickly in distilled water. 



5. Differentiate with 1% acetic acid, controlling by examination 

 under the microscope, until the amyloid appears reddish in 

 colour. 



6. Wash in distilled water. 



7. Mount directly in Aquamount. 



Results: 



Amyloid substance is stained red, while cells and nuclei are in 

 varying shades of blue. 



Notes: 



[a) The technique is not suitable for material which has under- 

 gone prolonged fixation in formalin. 



{b) The stain is extracted from the amyloid by alcohol. 



