SECTION TWO 



3. If mercuric chloride has been used for fixation treat sections 

 for the removal of mercuric precipitate by the standard method. 



4. Take sections down to distilled water. 



5. Immerse for two minutes in the methyl violet pyronin 

 (Solution A above). 



6. Pour off excess stain and carefully wipe the slide dry. 



7. Flood the preparation with acetone-orange G solution. 



8. Pour off after a few seconds. 



9. Flood the preparation with a fresh lot of acetone-orange G 

 solution and pour off after a few seconds. 



10. Wash quickly in pure acetone. 



1 1 . Rinse with two lots of xylol. 



12. Mount in balsam. 



Results: 



Cytoplasm, red; chromatin, violet; keratin, violet; connective 

 tissue, yellow. 



Reference: Bonney (1908). 



METHYLENE BLUE - BASIC FUCHSIN 

 Rapid method of demonstrating Negri bodies in sections 



Solutions required: 



A. Methylene blue . . 

 Basic fuchsin 

 Methyl alcohol, pure 

 Glycerine, pure . . 



B. Potass, hydroxide N.20 

 Distilled water 



C. Solution A 

 Solution B 



I gm. 

 1-75 gm. 

 100 ml. 

 100 ml. 



6-2£; ml. 

 9375 ml. 



10 ml. 

 025 ml. 



Note: Solution C should be freshly prepared as 

 required. 



301 



