STAINING, PRACTICAL AND THEORETICAL 



8. Rinse quickly with distilled water. 



9. Immerse in the mucicarmine solution for forty-five minutes. 



10. Rinse quickly with distilled water. 



11. Rinse quickly with 95% alcohol. 



12. Dehydrate rapidly but thoroughly with absolute alcohol. 



13. Clear in xylol and mount. 



Results: 



Mucin is stained red, while connective tissue is yellow, and 

 nuclei are black. 



Reference: Conn, H. J. & Darrow, M. A. (1947), pt. i, Sect. B, p. 14. 



MUCICARMINE (Mayer) 



Solution required: 



Mucicarmine, stock solution . . i volume 

 Alcohol 70% . . . . . . 10 volumes 



Note: This diluted solution should be freshly 

 prepared. 



Technique: 



Tissues should be fixed in absolute alcohol for five to eight 

 hours and embedded in paraffin wax, Celloidin or L.V.N. 



1. Bring paraffin sections down to distilled water; then stain 

 for ten to twenty-five minutes in the above solution. 



2. Wash rapidly with distilled water. 



3. Dehydrate with 70%, 95% and absolute alcohol. 



4. Paraffin sections are cleared in xylol; Celloidin or L.V.N, 

 sections in terpineol or origanum oil. 



5. Mount in Clearmount. 



Results: 



Mucin is stained red. 



Reference: Mayer, P. (1896). 



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