SECTION TWO 



MUCICARMINE (Southgate) 



This is used in accordance with the Mayer technique, but i vol- 

 ume of the stock solution is diluted with 9 volumes of distilled 

 water instead of 10 volumes 70% alcohol. 



Southgate's modification gives more uniform results than Muci- 

 carmine prepared by Mayer's original recipe. 



Reference: Southgate, H. W. (1927). 



Technique: 



1 . Material is fixed in absolute alcohol ; cleared ; and embedded 

 in paraffin wax. 



2. Sections are stained for ten minutes in the above solution. 



3. Wash with distilled water. 



4. Dehydrate by plunging the slide into two or three changes 

 of 95% alcohol. 



5. Pass through absolute alcohol ; then clear in xylol ; mount. 



Results: 

 Mucus is stained blue, while the remainder is colourless. 



Reference: Mayer, P, (1896). 



NADI REACTION 

 For oxidase granules 



Solutions required: 

 A. a-Naphthol . . . . , . i gm. 



Distilled water . . . . . . 100 ml. 



Place in a 250-ml. flask and boil until the a-Naphthol 

 begins to melt; then add 40% potassium hydroxide 



313 



