SECTION TWO 



NAPHTHOL GREEN B - HAEMATOXYLIN 

 For connective tissue 



Solutions required: 



A. Weigert's haematoxylin, A. 



B. Weigert's haematoxylin, B. 



C. Eosin, yellowish, i% in tap water. 



D. Ferric chloride, hydrated io%. 



E. Naphthol Green B, i% aqueous. 



F. Equal volumes of acetone and xylol. 



Technique: 



1. Paraffin sections are mounted on the slide and brought down 

 to distilled water in the usual manner. 



2. Stain for six minutes in a freshly prepared mixture consisting 

 of equal volumes of Weigert's haematoxylin A and B. 



3. Wash thoroughly in tap water; then stain for three minutes 

 in the eosin solution. 



4. Wash in tap water ; then immerse in the ferric chloride solu- 

 tion for five minutes. 



5. Rinse well in distilled water; then stain for five minutes in 

 the naphthol green solution. 



6. Differentiate for two or three minutes in 1% acetic acid. 



7. Drain well; then dehydrate with acetone, afterwards clear- 

 ing in acetone-xylol (as above); then mount. 



Results: 



Connective tissue, green; muscle and cytoplasm, pink. 

 Reference: Lillie, R. D. (1945). 



NAPHTHOCHROME GREEN 

 For the histochemical detection of beryllium 



Solutions required: 



A. Naphthochrome green G . . 0-25 gm. 

 (Michrome No. 938) 



L* 317 



