SECTION TWO 



Notes: 



{a) This histochemical test was developed by Denz (1949) 

 during an investigation of the toxic action of berylHum salts on 

 experiment animals, and the technique was reported at the time 

 as an example of the application of chemical methods to histo- 

 logical problems. 



{b) The original paper, which should be consulted for more 

 detailed information, deals with the effect of solubility of beryllium 

 compounds, intravenous injections in animals, utilization of the 

 method, etc. 



[c) In the original paper Denz describes the dye as naphtho- 

 chrome green B, Schultz No. 851; but the structural formula, 

 the chemical name, and the Schultz reference he gives all relate 

 to naphthochrome green G, not B. In fact, as far as I am aware, 

 and this has been confirmed by searches through dyestuffs 

 literature, there is no dye under the name of naphthochrome 

 green B, and Schultz No. 851 relates to an anthraquinone dyestuff, 

 known as alizarin direct blue B, according to the Colour Index 

 (1924), but not according to the new edition. Naphthochrome 

 green G is a triarylmethane dye. 



Reference: Denz, F. A. (1949). 



a-NAPHTHYL ACETATE - FAST BLUE B SALT 



For the demonstration of esterase activity in leucocytes in 

 human blood and bone marrow 



Solutions required: 



A. 10% Formalin in normal saline 



B. Phosphate buffer (M/20), pH 7-4 



C. a-Naphthyl acetate 1% in 50% 



acetone 



D. Solution B . . . . . . 40 ml. 



Solution C . . . • . . o-S ml. 



319 



