SECTION TWO 



7. Stain for one minute in Orange G solution (prepared as 

 above). 



8. Rinse thoroughly in 95% alcohol to remove all excess stain. 



9. Stain for two minutes in Papanicolaou stain ; then rinse five 

 to ten times in each of three jars of 95% alcohol. 



10. Rinse in absolute alcohol ; clear in xylol and mount. 



Results: 



Nuclei: violet. Erythrocytes: orange. Cornified cells: red, pink 

 or orange. Basophile cells : blue or green. 



Reference: Papanicolaou, G. N. (1942). 



PAPANICOLAOU STAIN EA36 



For paraffin sections of keratinizing epithelia 



(After P. L. Johnson & M. N. Klein, 1956) 



M 



Mix well, then filter 

 341 



