SECTION TWO 



(b) It is Stated in the original paper, to which readers are 

 referred, for further information, that the enhanced nuclear stain 

 and better cytoplasmic differentiation renders tissue morphology 

 more conspicuous and more readily interpreted than with the 

 routine haematoxylin and eosin methods. 



(c) Further application of the procedure to cyclic changes in 

 the vaginal mucosa as well as to pathological lesions are being 

 investigated by the authors (Johnson & Klein), 



(d) Readers are also referred to Aascher, Turner & De Boer's 

 methods, in which Papanicolaou stain and MF4 stain are used 

 for paraffin sections of vaginal epithelium. 



References : 



Johnson, P. L. & Klein, M. N. (1956). 



Papanicolaou, G. N. (1942). 



Aascher, A. W., Turner, C. J. & De Boer, C. H. (1956). 



Gurr, E. (i958«), pp. 19-23. 



PARALDEHYDE FUCHSIN - HAEMALUM - PICRO 

 INDIGOCARMINE 



For elastic, collagenous and muscle fibres, etc. 



Solutions required: 



A . Gomori 's fluid 



Potassium permanganate, 2-5% 



aqueous . . . . . . 12-5 ml. 



Sulphuric acid, 5% aqueous . . 12-5 ml. 



Distilled water . . . . . . 75 ml. 



B. Potassium metabisulphite, 2% 



aqueous 



C. Haemalum (Mayer) 



D. GoniorVs paraldehyde fuchsin (stock solution) 



i. Shake i gm. basic fuchsin with 200 ml. distilled water, at 



room temperature, in a flask, 

 ii. Raise to boiling point and allow the solution to boil for one 



minute; then allow it to cool to room temperature, 

 iii. Filter; then add 2 ml. each of concentrated HCl and 



paraldehyde. Shake well; then plug the neck of the flask 



with non-absorbent cotton wool. 



343 



