SECTION TWO 



Notes: 



In the same paper Gabe describes a similar procedure for 

 demonstrating alpha and beta cells of the pancreas as well as 

 neurosecretions in arthropods and vertebrates. 



Reference: Gabe, M. (1953). 



PARALDEHYDE - NEW FUCHSIN 



For the demonstration of beta cells in pancreatic islets 



and their tumours 



Solutiojis required: 



A. Chromic chloride, A.R. . . 5 gm. 

 Glacial acetic acid . . . . 5 ml. 

 Distilled water . . . . . . 100 ml. 



Note: This solution cannot be relied upon after 

 six months. 



B. Paraldehyde new fuchsin* 



New fuchsin (Michrome No. 624) i gm. 



Alcohol, 70% 100 ml. 



Dissolve; cool to room temperature, then add the 

 following mixture : 

 HCl, cone. . . . . . . 2 ml. 



Paraldehyde . . . . . . 2 ml. 



Shake well, then allow the stain to remain at 

 room temperature for twenty-four hours, or until the 

 solution is a deep purple colour. 



Technique: 



1. Material should be fixed in Bouin's fluid or in bufi^ered 

 neutral 10% formalin. 



2. Cut sections at 5//. or less and fix them to slides as usual. 



3. Remove paraffin wax with xylol, then pass through the 

 usual descending grades of alcohol into distilled water. 



4. Mordant in solution A for 72 hours. 



5. Rinse thoroughly in distilled water. 



* The solution, which is unreliable after about a week, should be stored in the 

 refrigerator. 



345 



