STAINING, PRACTICAL AND THEORETICAL 



Results. 



Collagen fibres: deep blue. Cytoplasm: red. Epithelial cells, 

 centroiles, basal bodies, nuclear structure : brilliant red. Erythro- 

 cytes : yellowish red. Connective tissue : blue. Secretory bodies : 

 varying according to their nature. Slime of goblet cells: azure 

 blue. Connective tissue wandering cells, smooth and striated 

 muscle: well defined. 



Reference: Pasini (1905). 



PERACETIC ACID - ORCEIN - HALMI STAIN 

 For connective tissues 



Solutions required: 

 A. Per acetic acid [Greenspan, 1946) 



Glacial acetic acid . . . . 95 -6 ml. 



Hydrogen peroxide, 30% . . 259 ml. 



Sulphuric acid, cone. . . . . 2-2 ml. 



Leave the solution to stand for one to three days; then add 

 0-04 gm. disodium hydrogen phosphate as a stabilizer. 

 Store in a refrigerator at 0° to 5°C. 



Note: Lillie (1954) states that he has kept such solutions in 

 the icebox for months, and a single Coplin jar of this reagent 

 may be used for eight to ten groups of nine slides before dis- 

 carding, but a positive control should be included, at least in 

 the later groups. 



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