SECTION TWO 



Acid salicylate 



0-17 gm. 



D. 



Dissolve the haematoxylin in 100 ml. of the water and place 

 in a flask of i litre capacity. Dissolve the ammonia alum in 

 200 ml. of the water and put aside. 



Measure out the glycerin and pour it into the flask containing 

 the haematoxylin solution. 



Use the alum solution now for rinsing out the glycerin 

 adhering to the inside of the measuring cylinder; then pour 

 the solution into the flask containing the haematoxylin. 



Dissolve the potassium iodate in about 20 ml. of the remain- 

 ing distilled water and add the solution to the haematoxylin. 



Shake the contents of the flask well to ensure thorough 

 mixing. Filter into the stock bottle. 



Shake the acid salicylate with the remaining 30 ml. or so of 

 the distilled water, and add to the stock bottle. 



Shake the bottle again thoroughly. The solution is ready for 

 immediate use. 



Light green SF yellowish 

 Distilled water 



E. Orange G 

 Distilled water 



F. Phosphotungstic acid 

 Distilled water 



G. Halmi stain, modified 

 Solution D 

 Solution E 

 Solution F 

 Acetic acid 1-3% aqueous 



Note: Solution G keeps for at least two weeks. 



. . 25 ml. 

 .. 75 drop 



H. Absolute alcohol, 95% 

 Glacial acetic acid 



Technique: 



1. Fix material either in Lillie's aqueous neutral calcium 10% 

 formalin for eighteen to twenty-four hours, or in 95% alcohol 

 for the same time. 



2. Decalcify mineralized tissues in 5% formic acid. 

 Embed in paraffin wax and cut sections at 6)a. 

 Fix sections to slides and remove paraffin wax with xylol. 

 Wash in absolute alcohol. 

 Oxidize in peracetic acid (solution A) for thirty minutes. 



3- 



4. 



5- 

 6. 



M' 



349 



