STAINING, PRACTICAL AND THEORETICAL 



7. Wash in running water for two minutes. 



8. Rinse in distilled water. 



9. Stain in the orcein solution in a covered jar for fifteen 

 minutes at 37° C. 



10. Differentiate in three changes of 70% alcohol for a total 

 time of five minutes. 



1 1 . Wash in distilled water. 



12. Stain in the Lillie-Mayer alum haematoxylin (Solution C) 

 for four minutes. 



13. Rinse in water. 



14. Differentiate in acid alcohol for thirty seconds. 



15. Blue in running tap water. 



16. Counterstain in the modified Halmi stain (solution G) for 

 twenty seconds. 



17. Rinse briefly in the acetic alcohol (solution H). 



18. Rinse in 95% alcohol. 



19. Dehydrate in absolute alcohol. 



20. Mount in Michrome mountant or Clearmount, or proceed 

 as follows : 



21. Clear in xylol. 



22. Mount in D.P.X. or Cristalite or Clearmount. 



Notes: 



{a) The material used by the author (Fullmer) comprised six 

 embryos ranging in age from two to six months as well as the upper 

 posterior alveolar processes of several individuals ranging from 

 four months to seventy-nine years in age. Also sections from 

 several extracted adult teeth were used. 



{b) It is claimed that the technique demonstrates elastic and 

 oxytalan fibres as well as fibrils in the mucous connective tissues 

 previously undescribed histochemically. Readers should consult 

 the original paper for further information. 



References : 



Fullmer, H. M. (i959)- 

 Lillie, R. D. (1954)- 

 Greenspan, F. P. (1946). 

 Halmi, N. S. (1952). 



