SECTION TWO 



14. Wash with 70% alcohol. 



15. Immerse in Feulgen's Fuchsin for ten to thirty minutes. 



16. Wash in running water for ten to thirty minutes. 



17. Stain in the celestin blue solution one half to three minutes. 



18. Rinse in water. 



19. Stain in the toluidine blue (solution H) for one half to three 

 minutes. 



20. Wash in running water for five minutes. 



21. Stain in the phosphomolybdic orange G (solution I) for 

 ten seconds. 



22. Wash in water for five to thirty seconds, until a yellow tinge 

 is just visible to the naked eye. 



23. Dehydrate through the usual graded alcohols. 



24. Clear in xylol and mount. 



Note : hi place of Toluidme blue, Iron Haematoxylin may he used 

 at step 19 in which case it will he necessary to differentiate quickly 

 before washing in running water {step 20). 



Results: 



Beta granules in the cyanophils and a number of granules and 

 vesicles in cells which stain as chromophobes by other methods, 

 are magenta to deep red : the colloid is magenta. Alpha granules 

 of acidophils, orange yellow, and erythrocytes are a shade more 

 yellow. 



Nuclei: are blue-black. 



Note: 



Compared with other methods, finer differences in the cytology 

 of the cyanophils can be appreciated. Cell counts can readily 

 be carried out, and the counts are more accurate, giving more 

 definite and clearer results than those obtained by older methods : 

 for instance cells appearing by Mallory and other histological 

 methods, to be chromophobes, are found to belong to cyanophil 

 series. 



The method has been applied with good cytological results to 

 the hypophysis of sheep and goats. 



Reference: Pearse (1950). 



357 



