SECTION TWO 



Notes: 



This is a modification of Zimmermann's method. The muci- 

 carmine employed in Zimmermann's technique is replaced by 

 periodic acid-Schiff reagent. 



References : 



Marks, I. N. & Drjsdale, K. M. (i957)- 

 Zimmermann (1925). 



PERIODIC ACID - SILVER - ORCEIN - ANILINE BLUE 



For the selective demonstration of elastin, reticulum and 



collagen 



(After G. L. Humason & C. C. Lushbaugh, i960) 



Solutions required: 



A. Pyridine, pure, redistilled 

 Alcohol, 95% 



B. Periodic acid 

 Distilled water 



C. Silver nitrate, 20% 



D. Sodium carbonate, 10% 



E. Solution C 

 Distilled water 



F. Ammonium hydroxide, specific 



gravity o-88o . . 

 Distilled water 



G. Solution D 

 Distilled water 



H. Solution E 



Add a little of solution F drop by drop, shaking the 

 flask after each addition until the white precipitate 

 formed just redissolves, leaving the solution slightly 

 opalescent. 



Add and shake in 10 ml. of solution G. 



Make up the volume to 100 ml. with distilled water. 



I. Solution F 0-5 ml. 



Distilled water 99-5 ml. 



359 



