STAINING, PRACTICAL AND THEORETICAL 



J. Formalin, 5% . . . . • • 50 ml. 



Solution D . . . . . . o-i ml. 



Note: This solution should be freshly prepared, 

 sufficient for one day's use. 



K. Gold chloride (brown or yellow) 0-5 gm. 

 Distilled water 



Sodium thiosulphate 

 Distilled water 



100 ml. 

 100 ml. 



M. Orcein . . . . . . . . i gm. 



Alcohol, 70% . . . . . . 100 ml. 



Hydrochloric acid, cone. . . o-6 to i ml. 



N. Phosphomolybdic acid, 5% aqueous. 



O. Koneff's aniline blue {Ko?teff, 1936, 

 modified) 



Aniline blue, water-soluble . . o-i gm. 



Oxalic acid . . . . . . 2 gm. 



Phosphomolybdic acid . . . . 15 gm. 



Distilled water . . . . . . 100 ml. 



Note: Dissolve the phosphomolybdic acid in the 

 water first ; add the oxalic acid and dissolve ; then the 

 aniline blue. 



P. Phosphomolybdic acid . . . . 5 gm. 



Distilled water . . . . . . 100 ml. 



Q. Acetic acid, glacial . . . . i ml. 



Distilled water . . . . . . 99 ml. 



Technique: 



1. Fix in a mercuric chloride-containing fluid for preference, 

 although satisfactory results can be obtained with material fixed 

 in 10% formalin. 



2. Embed in paraffin wax and cut sections at 6 to lO/x. 



3. Attach sections to slides with glycerin albumen or Haupt's 

 fixative. 



4. Remove paraffin wax with xylol. 



5. Wash in absolute alcohol. 



6. Wash in 95% alcohol. 



7. Immerse in pyridin-alcohol (solution A) for fifteen minutes. 



8. Rinse in 95% alcohol for two to three seconds. 



360 



