SECTION TWO 



(/) For more detailed information and colour plates, the 

 original paper should be consulted. 



Reference: Humason, G. L., Lushbaugh, C. C. (i960). 



PEROXIDASE REACTION 

 For blood films 



Technique: 



1. Air-dried blood films are flooded with Solution A for one 

 minute. Pour off ; then without washing and while still wet : 



2. Flood with Solution B and leave for two minutes ; then rinse 

 in tap water. 



3. Counterstain in Solution C for one minute. 



4. Wash with tap water; blot and dry. 



Results: 



Peroxidase granules, blue. Nuclei of leucocytes, orange-red. 



Note: Unmounted specimens keep for many months without 

 fading. 



Reference: Sato, A. & Shoji, K. (1927)- 



PHLOXIN - CHROME HAEMATOXYLIN 

 For differentiating cells of pancreatic islets 



Solutions required: 



A. Potassium permanganate, 1% 

 aqueous 



363 



