SECTION TWO 



19. Clear in xylol, and mount in D.P.X., or Cristalite on 

 Canada balsam. 



Results: 



Alpha cells: red. Beta cells: blue. Delta cells are stained pink 

 to red and are indistinguishable from alpha cells. Acinar zymogen 

 granules: red to colourless in pancreas. In hypophysis, the alpha 

 cells are pink; beta cells greyish blue, and not easily distin- 

 guishable from chromophobes. Nuclei reddish purple to bluish 

 violet. Erythrocytes: deep pink. Smooth muscle: pink. Col- 

 lagen is unstained. Goblet cell mucin appears coarsely granular 

 and dark, stained slightly greenish-blue. 



Note: The method is said to afford easy distinction of alpha 

 and beta granules in pancreatic islets on material fixed twelve 

 hours post mortem. 



Reference: Bell, E. T. (1946). 



PHLOXIN - HAEMATOXYLIN 

 For hyaline 



Solutions required: 



A. Ehrlich haematoxylin solution. 



B. Phloxin 0.5% in 25% alcohol. 



C. Lithium carbonate o-i% aqueous. 



Technique: 



1. Stain in Ehrlich haematoxyhn for five minutes. 



2. Wash in water; then stain one half to one minute in the 

 phloxine solution. 



3. Wash in tap water; then decolorize in the lithium carbon- 

 ate solution. 



4. Wash in tap water ; then dehydrate in the usual manner and 

 mount in balsam. 



Results: 



Fresh hyaline appears as red droplets and threads ; while older 

 hyaline is pink to colourless; nuclei, blue. 



Reference: Mallory, F. B. (1938), p. 207. 



