STAINING, PRACTICAL AND THEORETICAL 



2. Immerse in the phosphomolybdic acid solution for fifteen 

 minutes. 



3. Rinse in solution B. 



4. Dip into solution D. 



5. Counterstain with the eosin solution for one to two minutes. 



6. Mount in glycerine jelly. 



Results: 



Positive areas are stained blue, whilst negative areas are red. 



Reference: Landing, B. H., Uzman, L. L. & Whipple, Ann (1952). 



PICRO FUCHSIN (VAN GIESON) - HAEMATOXYLIN 

 For collagen and connective tissue 



Solutions required: 



A. Ehrlich haematoxylin 



B. Van Gieson stain (picro fuchsin) 



Technique: 



1. Paraffin sections are mounted on slides and brought down to 

 distilled water as usual. 



2. Stain in Ehrlich haematoxylin for five to thirty minutes. 



3. Blue in tap water in the usual manner; then examine while 

 still wet, under the microscope: the nuclei should be dark blue, 

 but if they are overstained or understained treat as described 

 under Haematoxylin (Ehrlich) - Eosin (Stage 4). 



4. Stain for three to five minutes with picro fuchsin ; then rinse 

 for a few seconds in water. 



5. Examine while still wet, under the microscope, and continue 

 the staining with picro fuchsin or continue the difterentiation 

 with water, whichever is necessary. 



6. Dehydrate as usual; clear in xylol; mount in D.P.X. or in 

 Cristalite, or Clcarmount. 



374 



