SECTION TWO 



Results: 



Nuclei of cells are stained dark brown to black. Collagen 

 fibres: bright red. Erythrocytes, muscle, epithelia and other 

 tissues are stained yellow. 



Note: 



If better colour contrast is desired in the nuclei it is recom- 

 mended that Weigert haematoxylin be used in place of Ehrlich. 



References : 



Van Gieson, J. (1889). 

 Carleton & Leach (1947). 



PICRO - GOMORI 



(After D. W. Menzies, 1959) 



A rapid and simple technique, being a modification of 



Mallory stain for the mass staining of pathological material 



for diagnostic and teaching purposes 



Solutions required: 



A. Haematoxylin (Weigert), A 



B. Haematoxylin (Weigert), B 



C. Alcohol, 95% 



Glacial acetic acid 



D. Picric acid, saturated, aqueous 



E. Solution D 

 Orange G . . 



F. Light green SF, yellowish, 10% 



aqueous 



G. Phosphotungstic acid, 10% 



aqueous 

 H. Chromotrope 2R, 10% aqueous 

 I. Acetic acid, 10% aqueous 

 J. Solution F 



Solution G 



Solution H 



Solution I 



Water 

 K. Acetic acid, 1% aqueous 



3 

 6 



6 



10 



ml. 

 ml. 

 ml. 

 ml. 



75 ml. 



375 



