SECTION TWO 



image which, according to the author, makes the technique a 

 reHable one for routine work more particularly in laboratories 

 where staining is carried out on a mass scale for teaching and 

 diagnostic purposes. 



(c) The author (Menzies) found that staining at the rate of 

 seventy to eighty slides a day, the solutions used in dishes holding 

 about 800 ml. each, required replacement every six to eight 

 weeks. The slides were carried from dish to dish in stainless steel 

 slotted racks. 



(d) The method had been in routine use for diagnostic pur- 

 poses in Menzies' laboratories for four years before the date of 

 the paper referred to above. 



(e) All the solutions listed above are stable. 



Reference: Menzies, D. W. (1959). 



PICRO ANILINE BLUE - EOSIN 

 For urinary casts 



Solutions required: 



A. Eosin yellowish 0-5% aqueous. 



B. Aniline blue 1% aqueous . . i ml. 

 Picric acid saturated, aqueous . . 10 ml. 

 Glycerine . . . . . . . . 10 drops 



Technique: 



1. The urine is centrifuged and the supernatant liquid decanted 

 as usual for microscopic examination. 



2. One drop of eosin solution is added to the sediment and 

 mixed by shaking from side to side for one to two minutes. 



3. Two drops of Solution B are added and mixed. The colour 

 of the sediment should now be distinctly blue-green; if it is red- 

 dish brown more of Solution B should be added till the blue-green 

 colour is obtained, but too much should be avoided. 



4. Some of the stained sediment is then transferred to a slide, 

 covered with a coverglass and examined. 



377 



