SECTION TWO 



PONTACYL BLUE BLACK SX-LUXOL FAST YELLOW 

 A general stain for animal tissues 



I gm. 

 lOO ml. 



2gm. 

 100 ml. 



30 ml. 

 10 ml. 



2gm. 

 100 ml. 



25 ml. 

 25 ml. 



2gm. 

 100 ml. 



Solutions required: 



A. Pontacyl blue black SX 

 Tap water 



B. Potassium dichromate 

 Distilled water 



C. Solution A 

 Solution B 



D. Chrome alum, cryst. (12H2O) 

 Tap water 



E. Solution A 

 Solution D 



F. Luxol fast yellow TN 

 Alcohol, 95% 



Technique: 



1. Fix pieces of material in 10% formalin. 



2. Dehydrate in graded alcohols, 



3. Clear in xylol. 



4. Embed in paraffin wax. 



5. Fix sections to slides and remove paraffin wax with xylol as 

 usual. 



6. Pass through the usual graded alcohols into distilled water. 



7. Stain in solution C or E for fifteen minutes. 



8. Wash in tap water. 



9. Differentiate in 70% alcohol until no more colour runs out 

 of the section. 



10. Counterstain in the luxol fast yellow (solution F) for five 

 minutes. 



11. Dehydrate with absolute alcohol. 



12. Mount in Clearmount or Michrome mountant or proceed 

 as follows: 



13. Clear in xylol. 



14. Mount in D.P.X. or Emexel or Clearmount or Michrome 

 mountant. 



N' 



381 



