SECTION TWO 



B. Chrome alum 

 Distilled water 



C. Solution A 

 Solution B 



D. Ammonium chromate 

 Distilled water 



E. Solution A 

 Solution D 



F. Luxol fast yellow TN 

 Alcohol, 95% 



2gm. 



100 ml. 



25 ml. 



25 ml. 



2gm. 



100 ml. 



25 ml. 



25 ml. 



2gm. 



100 ml. 



Technique: 



1. Fix tissues in 10% formalin. 



2. Dehydrate through the usual ascending grades of alcohol. 



3. Clear in xylol. 



4. Embed in paraffin wax. 



5. Fix sections to slides and remove paraffin wax with xylol as 

 usual. 



6. Hydrate through the usual descending grades of alcohol. 



7. Stain in solution C for fifteen minutes, or in solution E for 

 one hour. 



8. Wash in tap water. 



9. Differentiate in 70% alcohol until no more colour runs out 

 of the section. 



10. Counterstain in luxol fast yellow (solution F) for five 

 minutes. 



11. Dehydrate with absolute alcohol. 



12. Mount in Clearmount or in Michrome mountant, or 

 proceed as follows: 



13. Clear in xylol. 



14. Mount in D.P.X. or Emexel or in Clearmount or Michrome 

 mountant. 



Results with solution C {nuclear stain) : 



Nuclei : deep purple to red. Collagen : brilliant yellow. Keratin : 

 purple. Cartilage: yellow. Secretion granules of pancreas: red. 



Results with solution E {nuclear stain) : 



Nuclei: pale violet. Cytoplasm: yellow to brown. Keratin: 

 purple. Collagen and cartilage: yellow. Secretion granules of 



383 



