SECTION TWO 



Results: 



Nuclei: stained red. Cornified cells: red, pink or orange. 

 Erythrocytes: orange. Basophile cells: blue or green. 



Reference: Gurr, E. (1956, 1958a). 



PROTARGOL - GALLOCYANIN 

 For nerve fibres, sheaths and cells 



Solutto?is required: 



A. Protargol (silver proteinate) .. 1% aqueous. 



(Prepared by sprinkling the protargol powder on the 

 surface of the water and leaving it to dissolve.) 



B. Protargol i % aqueous . . 50 ml. 

 Alcohol 95% . . . . • • 50 ml. 

 Pyridine pure . . . . . . 0-5 ml. 



Note: The quantity of pyridine may be varied between 

 O'l ml. and 2 ml. The higher concentrations facilitate 

 the staining of thin fibres, whereas cell bodies and den- 

 trites are better demonstrated with the lower proportions 

 of pyridine. 



C. Boric acid 

 Sodium sulphite anhydrous 

 Hydroquinone . . 

 Acetone . . 

 Distilled water . . 



Dissolve each reagent in the above order in the water 

 adding the next only after the previous one has been dis- 

 solved entirely. 



D. Brown gold chloride . . . . 0-2 gm. 



Distilled water . . . . . . 100 ml. 



Glacial acetic acid . . . . i ml. 



Note: Solutions A, B, C and D must be stored in 

 dark bottles. 



E. Oxalic acid 2% aqueous. 



F. Sodium thiosulphite 5% aqueous. 



G. Gallocyanin solution (Einarson). 

 H. Phosphotungstic acid 5% aqueous. 



385 



