SECTION TWO 



Results : 



Nerve fibres and neurofibrils: blue-black. NissI bodies: pale 

 blue. Nuclei: blue-black with silver and gold if a higher percen- 

 tage of pyridine was used in solution. Myelin sheaths: bright 

 yellow. Connective tissues: various shades of blue and green. 



Reference: Foley, J. C. (1943). 



PURPURIN 

 For calcium deposits in pathological tissues 



Solutions required: 



A. Purpurin, saturated in absolute alcohol (about 0-7%). 



B. Sodium chloride, reagent grade, 0-75% aqueous. 



Technique: 



1. Fix material in 90% alcohol. 



2. Dehydrate; clear; embed in paraffin wax. 



3. Fix sections to slides; dewax and pass through the usual 

 descending grades of alcohol to distilled water. 



4. Stain in the purpurin solution for about ten minutes. 



5. Immerse in sodium chloride solution for about five minutes. 



6. Rinse with 70% alcohol until the stain ceases to come away 

 in clouds. 



7. Rinse with 90% alcohol and dehydrate with absolute alcohol. 



8. Clear in xylol and mount in balsam. 



Results: 



Calcium deposits are stained red. 



References : 



Grandis, V. & Mainini, C. (1900). 

 Gurr, E. (1958a). 

 Schmorl, C. G. (1928). 



PYRONIN - ALPHANAPHTHOL 



(After Graham) 



For oxidase granules in blood smears 



Solutions required: 

 A. a-Naphthol, pure . . . . i gm. 



387 



