SECTION TWO 



Technique: 



1. Tissues are fixed in neutral formalin io%, or in absolute 

 alcohol, and embedded in paraffin wax or Celloidin in the usual 

 manner. 



2. Bring sections down to distilled water, then immerse them 

 from two to forty-eight hours in the ammonium sulphide solution. 



3. Rinse thoroughly in distilled water. 



4. Counterstain in the basic fuchsin solution for five to twenty 

 minutes. 



5. Wash in water; drain well; rinse in 80% alcohol. 



6. Differentiate and dehydrate in absolute alcohol; clear in 

 xylol and mount in Canada balsam or in Cristalite or D.P.X. 



Results: 



Haemosiderin, dark brown to black. 

 Reference: Quincke, H. J. (1880). 



RAFFERTY'S STAIN 

 (Light Green-Haematoxylin) 



A general purpose stain 



Solutions required: 



A. Haematoxylin (Ehrlich). 



B. Light green (Michrome No. 240) . . 0-125 g"^- 

 Water 100 ml. 



Technique: 



1. Stain sections in solution A for five to ten minutes. 



2. Wash in tap water until blue. 



3. Stain in solution B for five minutes. 



4. Wash in water. 



5. Dehydrate through the usual graded alcohols. 



6. Clear in xylol and mount as usual. 



389 



