SECTION TWO 



Results: 



Basophile protoplasm: blue. Chromatin: violet blue. Nucleoli: 

 red. Connective tissue: faintly stained yellowish red. Muscle: 

 yellowish red. Erythrocytes: bright red. Acidophile granules of 

 leucocytes: bright red. Hyalin and granules of Russell: bright 

 red. Nuclei of the small lymphocytes are faintly stained violet. 



Reference: Houcke, E. (1928). 



RESORCIN - FUCHSIN STAIN SIMPLIFIED 



(After M. Krutsay, i960) 



For elastic fibres and as a substitute for SchifF's reagent in 



histology 



Solutions required: 



B. 

 C. 



D. 



E. 



m 



96% 



100 ml. 



2gm. 



0-5 ml. 



1-5 ml. 



A. Basic fuchsin, 

 alcohol . . 

 Resorcinol. . 

 HCl, cone. 

 Distilled water 

 Formaldehyde, 40% 

 Solution A \ equal 

 Solution B j volumes 



Note: Solution A will remain stable for several 

 months, but solution C should be prepared as and 

 when required for immediate use. 

 Picric acid, saturated, aqueous 

 Periodic acid, 0-5% aqueous 



TECHNIQUE I 



For elastic tissues 



1. Paraffin sections of formalin-fixed material are fixed to slides 

 and dewaxed in the usual manner. 



2. Wash in absolute alcohol. 



3. Stain in solution C for thirty minutes. 



4. Rinse in tap water. 



5. Wash in 96% alcohol for one to two minutes to remove 

 excess stain. 



391 



