STAINING, PRACTICAL AND THEORETICAL 



6. Rinse in distilled water. 



7. Counterstain in the picric acid solution for two minutes. 



8. Wash in 96% alcohol for one minute. 



9. Dehydrate in alcohol. 



10. Clear in xylol and mount in Canada balsam in xylol or 

 D.P.X. 



Results: 



Elastic fibres : deep purple to violet. Nuclei : pale red. Cartilage : 

 matrix: violet. Cytoplasm: yellow. 



TECHNIQUE II 



For use after periodic acid oxidation 



1. Fix paraffin sections to slides and dewax in the usual manner. 



2. Pass sections through to usual graded alcohols down to 

 distilled water. 



3. Oxidize in the periodic acid solution for twenty minutes. 



4. Wash in tap water for five minutes. 



5. Wash in 96% alcohol for one to two minutes. 



6. Stain in solution A (basic fuchsin) for twenty minutes. 



7. Rinse in tap water. 



8. Wash in 96% alcohol for one to two minutes. 



9. Dehydrate in absolute alcohol. 



10. Clear in xylol and mount. 



Results: 



The following are strongly stained purplish red: 



Thyroid and hypophyseal colloids, mucin, goblet cells, sarco- 

 lemma, basement membranes, glycogen, lipofuscin, hypophyseal 

 basiphil cell granules, corpora amylacea, fungi, calcareous 

 deposits, recent arterial hyalin and hyalin droplets and casts of 

 renal tubules. 



The following are stained moderately red: 



Fibrin, connective tissue fibrinoid, amyloid, brush borders, 

 and cartilage matrix. 



The following are stained pink: 



Collagen, reticular tissue, neuroglia, and myelin sheaths. 



392 



