SECTION TWO 



The following are unstained: 



Cytoplasm, chromatin, muscle, elastic fibres, erythrocytes, and 

 keratin. 



TECHNIQUE III 



For use after acid hydrolysis 



1. Fix the sections to slides and remove the paraffin wax with 

 xylol. 



2. Pass through the usual descending grades of alcohol into 

 water. 



3. Hydrolyse in N/i HCl at 58° C. for twelve minutes. 



4. Rinse in tap water. 



5. Stain in solution A (basic fuchsin) for twenty minutes. 



6. Rinse in tap water. 



7. Wash in 96% alcohol for one to two minutes. 



8. Dehydrate in absolute alcohol and clear in xylol. 



9. Mount in D.P.X. or Clearmount or Cristalite or if not 

 available, in neutral Canada balsam in xylol. 



Results: 



Chromatin: purplish red. Other tissues: colourless. 

 Reference: Krutsay, M. (i960). 



Notes: 



The original author (Krutsay) used phenol-xylene and xylene 

 in his dehydrating-clearing sequence, but if absolute alcohol is 

 available, phenol-xylene should not be essential. 



ROSE BENGAL - PHLOXIN 



For staining and counting eosinophils in canine hlood 



(After C. Cueto & J. H. U. Brown, 1958) 



Solutions required: 



A. Rose bengal 2B . . . . . . 10 gm. 



Tap water . . . . . . 100 ml. 



B. Phloxin B 10 gm. 



Tap water . . . . . . 100 ml. 



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