STAINING, PRACTICAL AND THEORETICAL 



It is conceivable, therefore, that the purple and violet shades 

 produced with Schiff's reagent may be due to molecular re- 

 arrangements involving methyl and/or ethyl groups. 



References : 



Bauer, H. (1933). 



Dodson, E. O. (1946). 



Feulgen, R. & Rossenbeck, H. (1924). 



Gurr, E. (1958). 



Lillie, R. D. (1951). 



Overend, W. G. & Stacey, M. (1949). 



Pearse, A. G. Everson (1953). 



Stowell, R. E. (1945). 



Tomasi, J. A. (1936). 



SHORE'S STAIN 

 For vaginal smears, keratin, etc. 



Technique: 



1. From the fixing solution, carry through alcohols to water; 

 stain in Solution A for two minutes ; then wash in running water 

 for five minutes. 



2. Stain for one minute in Solution B; then wash in water. 



3. Mordant in Solution C for one minute; then wash in water. 



4. Stain in Solution D for two minutes ; then without washing : 



5. Differentiate for one minute in 1% acetic acid. 



6. Dehydrate in the usual manner; clear in xylol and mount in 

 Clearmount. 



402 



