SECTION TWO 



Note: Solution A may be omitted in certain cases, as in the 

 routine treatment of menopause with oestrogens. With this omis- 

 sion smears can be stained in five minutes. 



Results: 



Cornified cells: orange-red. Non-cornified cells: green. 



Reference: Shorr, E. (1940). 



SHORR'S STAIN 



(Single solution) 



For vaginal smears 



Solutions required : 



A. Harris Haematoxylin 



B. Shorr's Stain (Single) 

 Technique: 



1 . Fix wet smears one to two minutes in equal parts of absolute 

 alcohol and ether: then pass into two changes of absolute alcohol, 

 followed by 80% and 66% alcohol. 



2. Rinse with distilled water for ten seconds ; then stain in 

 solution A for two minutes. 



3. Wash in running water for five minutes; then stain with 

 solution B for two minutes. 



4. Dehydrate with 66%, 80% and absolute alcohol; then clear 

 in xylol and mount. 



Results: 



Cornified cells: orange-red. Non-cornified cells: green. 



Reference: Shorr, E. (1941). 



SILVER CARBONATE - ORCEIN - ANILINE BLUE - FAST 



GREEN 



For demonstrating reticulin, elastin and collagen in the 



same tissue sections 



Solutions required: 



A. Celloidin 0-5% in equal vols, of ether and 

 absolute alcohol. 



403 



