STAINING, PRACTICAL AND THEORETICAL 



B. Pot. Permanganate 0-25% aqueous. 



C. Oxalic Acid 5% aqueous. 



D. Silver carbonate, Hortega [Foot's modification) 

 Silver nitrate 10% aqueous . . 10 ml. 

 Lithium carbonate, saturated 



aqueous . . . . . . . . 10 ml. 



Shake well; then allow to stand for ten 

 minutes or so in a 25 ml. measuring cylinder. 



Pour off the supernatent fluid, then transfer the precipitate to 

 a 100 ml. measuring cylinder, and add about 75 ml. distilled water, 

 shake well ; allow to settle ; then pour off the fluid and add a second 

 lot of distilled water. This process should be repeated three or 

 four times. 



Finally add 25 ml. of distilled water to the precipitate and add 

 28% ammonia solution drop by drop until the precipitate is almost 

 dissolved. 



Make up to 100 ml. with 90% alcohol. 



Filter and warm to 50° C. for 15 minutes before using. 



Technique: 



I. Material should be fixed in 10% formalin and embedded in 

 paraffin wax by the standard technique. 



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