SECTION TWO 



2. Sections 4 to 5/x in thickness are fixed to slides, dewaxed and 

 immersed in xylol for five minutes. 



3. Wash and immerse in absolute alcohol for two minutes. 



4. Immerse in solution A for five minutes (Celloidin). 



5. Drain slides for one minute. 



6. Immerse in 80% alcohol for five minutes. 



7. Rinse in water. 



8. Immerse in solution B (Pot. perman.) for five minutes. 



9. Rinse in water. 



ID. Immerse in solution C (oxalic acid) for five minutes. 



1 1 . Rinse in tap water. 



12. Wash in distilled water. 



13. Immerse in the silver carbonate solution in an oven 

 at 50° C. for ten to fifteen minutes (Soln. D). 



14. Rinse in distilled water. 



15. Immerse in solution E (20% neutral formalin) for five 

 minutes. 



16. Rinse in tap water. 



17. Tone in solution F (gold chloride) for five minutes. 



18. Rinse in tap water. 



19. Immerse in solution G for two minutes. 



20. Rinse in tap water. 



21. Stain in solution H (Orcein) for ten to fifteen minutes in 

 an oven at 37° C. or for one hour at room temperature. 



22. Rinse in 70% alcohol, followed by tap water. 



23. Stain in solution I (Aniline blue) for twenty to forty seconds 

 or solution J (Fast green) for ten to twenty seconds. 



24. Wash with 95% alcohol for six to eight seconds. 



25. Rinse briefly with absolute alcohol. 



26. Rinse with a mixture consisting of equal volumes of xylol 

 and absolute alcohol until clear. 



27. Rinse with several changes of xylol, and mount. 



405 



