STAINING, PRACTICAL AND THEORETICAL 



Results: 



With Picro aniline blue: 



Elastic fibres reddish brown 



Collagen blue 



Reticulum black 



Muscle blue-green 



Nuclei tan to brown 



Erythrocytes yellowish tan 



Cytoplasm pale blue 



With Picro fast green : 



Elastic fibres orange to reddish brown 



Collagen blue-green 



Reticulum black 



Muscle green 



Nuclei tan to brown 



Erythrocytes yellow to orange 



Cytoplasm light green 



Reference: Lewis, A. L. & Jones, R. S. (1951). 



SILVER NITRATE - GOLD CHLORIDE - PARACARMINE 



(Da Fano) 



For Golgi apparatus 



A. Cobalt nitrate 1% aqueous . . 100 ml. 

 Formalin . . . . . . • • 15 rnh 



B. Silver nitrate 1-5% aqueous. 



{N.B. : This should be stored in an amber or blue 

 glass bottle.) 



C. Cajal's Reducer. 



Hydroquinone 2% aqueous . . 100 ml. 

 Neutral formalin . . . . . . 15 ml. 



Sodium sulphite anhydrous . . 0-5 gm. 



N.B.: This solution should be freshly prepared. 



D. Gold chloride o-2% aqueous. 



E. Sodium thiosulphate 5% aqueous. 



F. Paracarmine (Mayer). 



406 



