118 BIOCHEMICAL STUDIES OF PHYLOGENY 



whilst for the other annelids mentioned the phosphagen was 

 always AP, in Nereis there appeared to be quite a lot of CP in 

 five out of the seven normal samples. Thus out of the 15-81% 

 due to total phosphagen the amount due to CP was 5-57%. The 

 authors considered that these values of CP were due to errors in 

 their technique and that creatine phosphate was not actually present. 

 In Sipunculus nudus one measurement was made from 1-46 g 

 of body wall. This gave a total phosphate level of 0*66 mg of 

 which 71 % was due to AP. 



(e) Cephalopoda 



In Sepia officinalis various parts of the animals were analysed 

 for phosphagen with the following results : 



Expt. 1 Expt. 2 Expt. 3 



In Octopus vulgaris the values from one animal came to, mantle 

 33%, funnel 27% and tentacle 12% phosphagen. It is of interest 

 that in both Octopus and Sepia some of the phosphagen was 

 apparently due to CP. In five out of the nine cases where phos- 

 phagen was present in Sepia there were traces of CP present, in 

 one case it being one-third of the total 6% due to phosphagen. 

 In Octopus in one case out of the three CP was present, it making 

 up half of the total 12% due to phosphagen. 



(f) Echinoderms 



Two measurements on the body wall of Cucumaria planci 

 showed no phosphagens to be present. However, two other 

 experiments on the phosphagens of the body wall of another 

 species of holothuriam, Synapta inhoerens, gave values of 5% and 

 25% of the total phosphate content as being due to phosphagen. 

 The phosphagen was AP. Nine experiments on the jaw muscles 

 from the Aristotle's lantern of Strongylocentrotus lividus gave values 

 from 42%-92% of the total phosphate as being due to phosphagen. 

 Analysis showed that about one-third or more of this phosphagen 

 was due to CP. 



